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Related Experiment Video

Updated: Jul 28, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Comprehensive Guideline for Microbiome Analysis Using R.

Joseph Boctor¹, Mariam Oweda², Mohamed El-Hadidi³

¹Biotechnology Program, American University in Cairo (AUC), Cairo, Egypt.

Methods in Molecular Biology (Clifton, N.J.)

|May 31, 2023

Summary

This summary is machine-generated.

This guide simplifies microbiome data analysis using R. It details essential R packages like phyloseq, DADA2, and Metacoder for efficient data integration and exploration.

Area of Science:

Microbiology
Bioinformatics
Computational Biology

Background:

Microbiome research generates complex datasets requiring specialized analytical tools.
Existing R packages for microbiome analysis are numerous, leading to a need for consolidated guidance.

Keywords:

DADA2 MegaR Microbiome data analysis R Packages microbiomeExplorer phyloseq

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