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Fiber and Electrical Field Alignment Increases BDNF Expression in SH-SY5Y Cells following Electrical Stimulation.

Quy-Susan Huynh1,2, R M Damian Holsinger1,2

  • 1Laboratory of Molecular Neuroscience and Dementia, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.

Pharmaceuticals (Basel, Switzerland)
|June 1, 2023
PubMed
Summary

This study shows that aligning nanofibers can help neuroblastoma cells produce brain-derived neurotrophic factor (BDNF). Combining this alignment with an electric field further enhances BDNF expression for neural regeneration.

Keywords:
electrical stimulationelectrospinningneurotrophic factors

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Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Tissue Engineering

Background:

  • Limited neurotrophic factor expression in neural tissue engineering scaffolds hinders sustained neural regeneration.
  • A localized and sustained delivery method for neurotrophic factors is crucial for effective neural repair.

Purpose of the Study:

  • To investigate the induction of trophic factor expression in neuroblastoma cells via electrical stimulation.
  • To explore the combined effects of electrospun nanofiber orientation and electrical fields on neurotrophic factor production.

Main Methods:

  • Human SH-SY5Y neuroblastoma cells were cultured on polycaprolactone electrospun nanofibers.
  • Cells were subjected to electrical stimulation (100 mV/mm electric field).
  • Analysis included nuclear morphology and brain-derived neurotrophic factor (BDNF) expression, considering fiber orientation and alignment relative to the electric field.

Main Results:

  • Nuclear deformation was primarily influenced by nanofiber orientation, not the electrical field.
  • Nanofiber orientation alone induced BDNF expression.
  • Combined nanofiber orientation (perpendicular to the electric field) and electrical stimulation significantly enhanced BDNF expression.

Conclusions:

  • Nanofiber orientation is a key factor in modulating cellular response and neurotrophic factor expression.
  • The synergistic effect of aligned nanofibers and electrical stimulation offers a promising strategy for enhancing BDNF production in neural tissue engineering scaffolds.
  • This approach could lead to improved methods for sustained neural regeneration.