Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and validation of nomograms for predicting overall survival and cancer-specific survival in female gastric signet-ring cell carcinoma: a SEER database analysis.

Translational cancer research·2026

Same author

Direct Lithium Extraction from a Complex Acidic Brine Using Aluminum Hydroxide.

ACS omega·2026

Same author

Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing.

Gels (Basel, Switzerland)·2026

Same author

TSDSNet: a three-branch detail-sensitive and noise-suppressed fusion network for label-free microscopic image enhancement.

Applied optics·2026

Same author

A Dual-Cascade Activatable Photoacoustic Sensor for Tracking Iron(II) and Reactive Oxygen Species during Ferroptosis.

Analytical chemistry·2026

Same author

Evaluation of Indigo Naturalis Prepared Using a Novel Method: Anti-Inflammatory Activities Against Colonic Cancer Cell Lines.

Mediators of inflammation·2026

Search research articles

Related Experiment Video

Updated: Jul 28, 2025

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

Published on: November 11, 2010

Laser speckle contrast imaging based on spatial frequency domain filtering.

Linjun Zhai¹, Yongzhao Du^1,2, Yuqing Fu²

¹School of Biomedical Science, Huaqiao University, Quanzhou, China.

Journal of Biophotonics

|June 1, 2023

Summary

This summary is machine-generated.

This study introduces a new spatial frequency filtering method to separate static and dynamic speckles, improving blood flow imaging by enhancing contrast and efficiency.

Keywords:

Fourier transform fluctuation modulation laser speckle contrast image spatial frequency domain filtering

More Related Videos

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Published on: August 5, 2009

Related Experiment Videos

Last Updated: Jul 28, 2025

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

Published on: November 11, 2010

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Published on: August 5, 2009

Area of Science:

Biomedical Optics
Image Processing
Laser Speckle Imaging

Background:

Laser speckle imaging is crucial for visualizing blood flow.
Separating static and dynamic speckles is challenging but essential for accurate flow quantification.
Existing methods may suffer from background noise and computational inefficiency.

Purpose of the Study:

To develop a novel method for effectively separating static and dynamic speckles.
To improve the accuracy and efficiency of 2-D blood flow mapping using laser speckle imaging.
To enhance image quality metrics such as contrast and signal-to-noise ratio.

Main Methods:

Utilized spatial frequency domain filtering (2D Fourier transform, low-pass, and high-pass filtering).
Processed raw speckle image sequences frame by frame.
Averaged filtered sequences in the time domain to obtain static and dynamic components.
Calculated time-averaged modulation depth for blood flow mapping.

Main Results:

Successfully separated static and dynamic speckle components.
Demonstrated effective suppression of background non-uniformity in phantom and in vivo experiments.
Achieved significant improvements in image contrast, contrast-to-noise ratio, and imaging dynamic range.
Showcased high computational efficiency of the proposed method.

Conclusions:

The proposed spatial frequency domain filtering method is effective for separating static and dynamic speckles.
This technique enhances 2-D blood flow imaging by improving image quality and reducing noise.
The method offers a computationally efficient and robust approach for biomedical applications.