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Published on: October 25, 2017
Fluorometric Determination of DNA Nanostructure Biostability
Hannah Talbot1, Bharath Raj Madhanagopal1, Andrew Hayden1
1The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
Abstract:
The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.
