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Fluorometric Determination of DNA Nanostructure Biostability
Hannah Talbot1, Bharath Raj Madhanagopal1, Andrew Hayden1
1The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
ACS Applied Bio Materials
|June 1, 2023
Summary
We developed a new assay to measure DNA nanostructure stability, crucial for advancing DNA nanotechnology. This method efficiently assesses DNA nanostructure biostability against enzymes and serum.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- DNA nanostructures are crucial for nanotechnology applications.
- Improving DNA nanostructure biostability is essential for their practical use.
- Existing methods for assessing biostability can be limited.
Purpose of the Study:
- To develop a novel, plate-compatible assay for measuring DNA nanostructure biostability.
- To utilize ethidium bromide as a fluorometric indicator for DNA integrity.
- To demonstrate the assay's utility by testing various DNA nanostructures.
Main Methods:
- A fluorometric assay using ethidium bromide was designed.
- The assay is compatible with standard microplate readers.
- Biostability was tested against nucleases and in fetal bovine serum.
Main Results:
- The assay successfully measured the biostability of duplex DNA, double crossover motifs, and DNA origami nanostructures.
- The method demonstrated scalability for high-throughput sample analysis.
- The assay provided quantitative data on DNA nanostructure degradation.
Conclusions:
- The developed assay is a robust and scalable tool for assessing DNA nanostructure biostability.
- This method can aid in the development of more stable DNA nanostructures for nanotechnology.
- The assay complements existing techniques, offering a valuable resource for the field.
