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Aptamer-based DNA nanoswitches for multiplexed protein detection
Sravya Kovvali1, Camryn A Beckles1,2, Arun Richard Chandrasekaran1,3
1The RNA Institute, University at Albany, State University of New York, Albany, NY, 12222 USA. khalvorsen@albany.edu.
Chemical Communications (Cambridge, England)
|July 31, 2026
Summary
Researchers developed simple, sensitive, and specific protein detection platforms using aptamer-based recognition and DNA nanoswitches. This novel approach enables dual detection of key blood proteins like thrombin and vascular endothelial growth factor (VEGF).
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Nanotechnology
Background:
- Protein detection is crucial for healthcare and research.
- Existing methods can be complex or lack sensitivity.
- Modular platforms offer adaptable solutions for diagnostics.
Purpose of the Study:
- To develop a simple, sensitive, and specific platform for protein detection.
- To combine aptamer-based recognition with DNA nanoswitches.
- To demonstrate dual detection of clinically relevant proteins.
Main Methods:
- Utilized aptamer-based recognition for target protein binding.
- Employed conformationally-responsive DNA nanoswitches for signal transduction.
- Integrated these components into a modular detection platform.
- Validated the platform using thrombin and vascular endothelial growth factor (VEGF) as model analytes.
Main Results:
- Achieved simple, sensitive, and specific detection of target proteins.
- Demonstrated successful dual detection of thrombin and VEGF.
- The DNA nanoswitch platform showed high responsiveness and specificity.
Conclusions:
- The developed platform offers a promising tool for protein detection in healthcare and research.
- Aptamer-DNA nanoswitch integration provides a versatile strategy for multiplexed diagnostics.
- This modular approach facilitates the detection of clinically relevant biomarkers.
