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Updated: Jul 27, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018
Valorization of clove (
Pramod Bandara1, Mahinda Senevirathne1
1Department of Food Science and Technology, Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka, P.O. Box 02, Belihuloya, Sri Lanka.
Clove by-products, specifically stem and leaf essential oils, show high potential as natural grain protectants against rice weevils. Clove stem essential oil demonstrated superior repellency and mortality, offering a sustainable alternative to synthetic insecticides.
Area of Science:
- Agricultural Science
- Entomology
- Natural Product Chemistry
Background:
- Clove (Syzygium aromaticum) by-products like stems and leaves are often underutilized.
- Post-harvest rice loss due to pests like rice weevils necessitates sustainable management strategies.
- Synthetic insecticides pose environmental risks and contribute to pest resistance.
Purpose of the Study:
- To evaluate the potential of clove stem and leaf essential oils as natural grain protectants against rice weevils.
- To analyze the chemical composition of these essential oils.
- To determine their repellent and insecticidal efficacy.
Main Methods:
- Essential oils were extracted from clove stems and leaves.
- Chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS).
- Contact repellent activity and fumigation mortality assays were conducted, calculating mortality percentage and LD50 values.
Main Results:
- Clove stem essential oil yielded higher (5.52%) than leaf essential oil (3.92%).
- Stem essential oil exhibited maximum repellency (100%) and mortality (96.67%) at 100 µL dose.
- The lowest LD50 (28.06 µL/L) was observed for stem essential oil, indicating higher toxicity. Major components identified include Eugenol and β-caryophyllene.
Conclusions:
- Clove by-products can be successfully valorized as effective grain protectants.
- Clove stem essential oil is a promising, eco-friendly alternative for managing rice weevils.
- This approach reduces reliance on synthetic insecticides and minimizes post-harvest losses.
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