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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
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Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Related Experiment Video

Updated: Jul 27, 2025

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

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Notch signaling pathway involved in

Mingxia Wang1, Zailing Shang1, Fei Qiao1

  • 1Basic Medical Institute of Ningxia Medical University, Yinchuan, China.

Frontiers in Cellular and Infection Microbiology
|June 5, 2023
PubMed
Summary

The Notch signaling pathway regulates dendritic cell responses to Echinococcus granulosus ferritin, impacting T-cell immunity. This pathway is a potential therapeutic target for E. granulosus infection.

Keywords:
Echinococcus granulosusNotch signalantigendendritic cellimmune responsematuration

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Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Area of Science:

  • Immunology
  • Cell Biology
  • Parasitology

Background:

  • The Notch signaling pathway is crucial for immune cell development and function.
  • Dendritic cells (DCs) play a key role in T-cell mediated immunity.
  • Echinococcus granulosus (E. granulosus) infection involves complex immune responses influenced by parasite antigens like ferritin and malate dehydrogenase (mMDH).

Purpose of the Study:

  • To investigate the role of the Notch signaling pathway in the development and differentiation of dendritic cells (DCs).
  • To determine how Notch signaling affects DC-mediated immune responses to E. granulosus antigens, specifically ferritin and mMDH.
  • To explore the involvement of the Notch pathway in E. granulosus infection.

Main Methods:

  • In vitro models were created using a Notch inhibitor (DAPT) and activator (Jagged1) to modulate Notch signaling in DCs.
  • DC development, differentiation, migration, T-cell proliferation capacity, and cytokine secretion were analyzed.
  • Changes in DC response to E. granulosus antigens and expression of key Notch pathway molecules were assessed.

Main Results:

  • Notch inhibition (DAPT) impaired DC maturation, reduced sensitization to E. granulosus ferritin, and weakened immune responses, including T-cell proliferation and co-stimulatory molecule expression.
  • Notch activation (Jagged1) partially restored DC function and enhanced responses to E. granulosus ferritin.
  • E. granulosus mMDH-stimulated DC responses showed minimal alteration by Notch pathway modulation.

Conclusions:

  • The Notch signaling pathway is significantly involved in the immune response mediated by DCs sensitized to E. granulosus ferritin.
  • Modulating the Notch pathway could offer a novel therapeutic strategy for treating E. granulosus infections.