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LysGR1, a novel thermostable endolysin from

Dahee Choi1, Minsuk Kong1

  • 1Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, Republic of Korea.

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|June 5, 2023
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Summary

A novel bacteriophage, GR1, and its endolysin LysGR1 were identified to combat Geobacillus stearothermophilus spoilage in canned foods. LysGR1 shows broad-spectrum lytic activity and thermostability, making it a promising biocontrol agent.

Keywords:
Geobacillus stearothermophilusbacteriophageendolysinenzymatically active domainfood spoilage

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Area of Science:

  • Microbiology
  • Bacteriology
  • Food Science

Background:

  • Geobacillus stearothermophilus causes spoilage in low-acid canned foods.
  • Bacteriophages offer potential solutions for controlling bacterial contamination.

Purpose of the Study:

  • To isolate and characterize a bacteriophage infecting G. stearothermophilus.
  • To evaluate the lytic activity and potential of its endolysin, LysGR1, as a biocontrol agent.

Main Methods:

  • Isolation and genomic characterization of phage GR1.
  • Biochemical and lytic activity assays of endolysin LysGR1 and its domains.
  • Biofilm removal and thermostability tests.

Main Results:

  • A novel Siphoviridae phage, GR1, was isolated from soil.
  • Its endolysin, LysGR1, possesses N-terminal amidase and C-terminal LysM domains.
  • LysGR1 demonstrated broad lytic activity against G. stearothermophilus and other foodborne pathogens, and effectively reduced biofilms.
  • LysGR1 and its enzymatically active domain (EAD) showed high thermostability.

Conclusions:

  • LysGR1 is a potent antimicrobial agent with broad lytic activity and biofilm eradication capabilities.
  • Its thermostability and efficacy suggest potential application as a biocontrol agent in the food industry.
  • Further research into LysGR1 and its EAD could lead to novel food preservation strategies.