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Suzana Hađina1, Branka Bruvo Mađarić2, Snježana Kazazić3

  • 1Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.

Frontiers in Microbiology
|June 5, 2023
PubMed
Summary

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This study identified Malassezia pachydermatis in brown bears, revealing four genotypes, including one new bear-specific strain. MALDI-TOF mass spectrometry proved effective for identifying subtle genotypic differences in this opportunistic pathogen.

Area of Science:

  • Microbiology
  • Genetics
  • Veterinary Medicine

Background:

  • Malassezia pachydermatis is a zoophilic yeast with invasive potential in humans.
  • Limited data exists on wild animal genotypes, despite its prevalence.
  • Brown bears (Ursus arctos) are a potential reservoir for this pathogen.

Purpose of the Study:

  • To identify and genotype Malassezia pachydermatis isolates from brown bears.
  • To evaluate MALDI-TOF mass spectrometry for yeast identification and differentiation.
  • To investigate the transmission potential and host adaptation of M. pachydermatis.

Main Methods:

  • Culture-based identification of 80 yeast isolates from 42 brown bears.
  • MALDI-TOF mass spectrometry (MS) for species identification and genotypic analysis.
Keywords:
MALDI-TOF mass spectraMalassezia pachydermatisUrsus arctosbrown bearmultilocus genotypingnovel genotypes

Related Experiment Videos

  • Genotyping using LSU, ITS1, CHS2, and β-tubulin markers.

    • Main Results:

    • M. pachydermatis was identified in brown bears.
    • Optimizing MALDI-TOF MS parameters (young biomass or lowered cut-off) improved species identification.
    • Four distinct genotypes were found, with one previously identified in dogs and a novel bear-specific genotype identified.

    Conclusions:

    • Malassezia pachydermatis circulates in wild brown bear populations.
    • MALDI-TOF MS can differentiate M. pachydermatis genotypes, aiding in tracking transmission.
    • The identification of a bear-specific genotype suggests adaptation to this host.