Response of
Jiaying Cheng1, Mengshi Xiao1, Xinmiao Ren1
1College of Food Science and Engineering, Ocean University of China, Qingdao, 266003 China.
Marine Life Science & Technology
|June 5, 2023
Summary
Fermenting alginate oligosaccharides (AOS) with chicken gut microbes creates compounds that inhibit Salmonella Typhimurium. This suggests AOS can be a feed additive to combat poultry pathogens by altering gut microbial metabolites.
Area of Science:
- Marine biotechnology
- Animal science
- Microbiology
Background:
- Alginate oligosaccharides (AOS) are functional feed additives from marine brown algae.
- Their impact on gut microbiota and microbial metabolites, particularly against poultry pathogens, is not well understood.
Purpose of the Study:
- To investigate the effect of AOS fermented by chicken fecal microbiota on Salmonella Typhimurium.
- To analyze the metabolic and transcriptomic changes induced by fermented AOS (F-AOS).
Main Methods:
- In vitro fermentation of AOS with chicken fecal inocula.
- Metabolomic and transcriptomic analyses (including RT-qPCR).
- High-performance liquid chromatography (HPLC) for metabolite quantification.
Main Results:
- AOS alone did not inhibit S. Typhimurium growth.
- Supernatant of fermented AOS (F-AOS) significantly reduced S. Typhimurium abundance in chicken fecal microbiota.
- F-AOS altered gene expression in S. Typhimurium, downregulating flagellar and type III secretion systems.
- F-AOS significantly changed 205 microbial metabolites, including increased indolelactic acid and 3-indolepropionic acid.
Conclusions:
- Fermentation of AOS by chicken gut microbiota generates antibacterial compounds.
- These compounds modulate S. Typhimurium virulence and metabolism.
- AOS show potential as a feed additive to control intestinal pathogens in poultry.
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