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Analysis of Fatty Acid Content and Composition in Microalgae
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Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

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Malaysian brown macroalga

Kogilavani Subermaniam1,2, Sze Yuen Lew1, Yoon Yen Yow3

  • 1Department of Anatomy, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.

Iranian Journal of Basic Medical Sciences
|June 5, 2023
PubMed
Abstract

Insights

Malaysian Padina australis extract (PAEE) reduced neuroinflammation in microglial cells by suppressing reactive oxygen species (ROS) and pro-inflammatory cytokines. This suggests P. australis as a potential therapy for neurodegenerative diseases.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Marine Biology

Background:

  • Neuroinflammation and microglial activation are key pathological features in central nervous system disorders.
  • Excess reactive oxygen species (ROS) and pro-inflammatory cytokines exacerbate neuronal damage during chronic microglial activation.
  • Padina australis, a brown macroalga, exhibits anti-neuroinflammatory properties, but its mechanisms are poorly understood.

Purpose of the Study:

  • To investigate the potential of Malaysian Padina australis ethanol extract (PAEE) in mitigating lipopolysaccharide (LPS)-induced neuroinflammation.
  • To elucidate the mechanisms underlying the anti-neuroinflammatory effects of P. australis in BV2 microglial cells.

Main Methods:

  • BV2 microglial cells were stimulated with lipopolysaccharide (LPS).
  • The effects of varying concentrations of P. australis ethanol extract (PAEE) on cell viability, nitric oxide (NO), prostaglandin E2 (PGE2), intracellular ROS, and inflammatory markers (iNOS, COX-2, TNF-α, IL-6) were assessed.
  • Liquid chromatography-mass spectrometry (LC-MS) was used to identify compounds within the PAEE.

Main Results:

  • LPS stimulation increased NO, PGE2, intracellular ROS, iNOS, COX-2, TNF-α, and IL-6 levels, while reducing cell viability.
  • PAEE treatment (0.5-2.0 mg/ml) dose-dependently reversed these LPS-induced effects.
  • LC-MS analysis identified methyl α-eleostearate, ethyl α-eleostearate, niacinamide, stearamide, and linoleic acid in PAEE.

Conclusions:

  • PAEE effectively suppresses LPS-induced neuroinflammation in BV2 microglial cells.
  • The anti-neuroinflammatory effects are attributed to the reduction of intracellular ROS and pro-inflammatory mediators.
  • Padina australis demonstrates therapeutic potential for neuroinflammatory and neurodegenerative diseases.
Keywords:
BV2 microglialBrown algaeCytokinesMajor compoundsNeuroinflammationOxidative damage

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