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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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Published on: November 16, 2019

On-Chip Mirror Enhanced Multiphoton Upconversion Super-Resolution Microscopy.

Yongtao Liu^1,2, Jiajia Zhou², Shihui Wen²

¹Smart Computational Imaging Laboratory(SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China.

|June 5, 2023

Summary

This summary is machine-generated.

Multiphoton upconversion super-resolution microscopy (MPUM) achieves higher resolution using nonlinear light. A novel on-chip mirror reduces excitation power by 10x, enabling super-resolution imaging with lower phototoxicity.

Keywords:

mirror enhanced multiphoton imaging self-interference stimulated depletion microscopy upconversion nanoparticles, on-chip super-resolution

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Area of Science:

Biomedical Optics
Microscopy
Nanotechnology

Background:

Multiphoton upconversion super-resolution microscopy (MPUM) offers enhanced resolution and penetration depth.
High excitation power in MPUM leads to increased phototoxicity, limiting its application.
Utilizing the transparent biological window with nonlinear near-infrared emission is key to MPUM.

Purpose of the Study:

To develop a method for achieving nonlinear saturation emission in MPUM under low excitation power.
To reduce phototoxicity associated with high excitation power in MPUM.
To enhance the excitation efficiency and resolution of MPUM.

Main Methods:

Introduction of a simply designed on-chip mirror to manipulate local electromagnetic fields.
Utilizing interference effects to confine the point spread function.
Implementing the on-chip mirror without adding complexity to the MPUM setup.

Main Results:

Achieved nonlinear saturation emission under significantly lower excitation power.
Reduced excitation power requirement by 10-fold.
Demonstrated a lateral resolution of approximately 35 nm (1/28th of excitation wavelength) in nanoparticle imaging.
Confirmed the effectiveness of the on-chip mirror in enhancing excitation efficiency.

Conclusions:

The on-chip mirror provides a simple and effective solution for super-resolution enhancement in MPUM.
This method significantly lowers the required excitation power, reducing phototoxicity.
The approach facilitates high-resolution imaging with predesigned on-chip devices.