Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunonephelometric assays.

E Ackerman, J W Rosevear

    Medical Progress Through Technology
    |January 30, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Nephelometry measures scattered light to analyze particles in immunoassays. While offering simplicity and precision, its sensitivity to noise limits current applications, though future developments may expand its use.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of altitude acclimatization on the equilibrium constant of rat oxymyoglobin.

    Project report. USAF School of Aviation Medicine·2014
    Same author

    Diffusion of n-butyl-p-aminobenzoate (BAB), lidocaine and bupivacaine through the human dura-arachnoid mater in vitro.

    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2000
    Same author

    Pathologic quiz case: recurrent hypercalcemia.

    Archives of pathology & laboratory medicine·2000
    Same author

    [Does incidence of hepatitis A increase during shmitah (the Sabbatical year)?].

    Harefuah·2000
    Same author

    Intrafamilial transmission of hepatitis C virus: a systematic review.

    Journal of viral hepatitis·2000
    Same author

    Preliminary evidence for aberrant cortical development in abused children: a quantitative EEG study.

    The Journal of neuropsychiatry and clinical neurosciences·1998
    Same journal

    A new computerized control unit for small bone surgical instruments.

    Medical progress through technology·1997
    Same journal

    Guinea pig auditory nerve response triggered by a high density electrode array.

    Medical progress through technology·1997
    Same journal

    Non-invasive assessment of systemic elastic behaviour in hypertensive patients: analysis of possible determinants.

    Medical progress through technology·1997
    Same journal

    Mechanical impedance of layered tissue.

    Medical progress through technology·1997
    Same journal

    Using movement parallax for 3D laparoscopy.

    Medical progress through technology·1996
    Same journal

    Acceleration plethysmography to evaluate aging effect in cardiovascular system. Using new criteria of four wave patterns.

    Medical progress through technology·1996
    See all related articles

    Area of Science:

    • Analytical Chemistry
    • Biochemistry
    • Immunology

    Background:

    • Nephelometry analyzes particle size, shape, and concentration via scattered light.
    • In immunoassays, antigen-antibody complexes serve as the scattering particles.
    • Nephelometry offers advantages in simplicity and precision over other assay methods.

    Purpose of the Study:

    • To review the principles and applications of nephelometry in immunoassays.
    • To discuss the limitations of current nephelometry techniques in immunological assays.
    • To explore future developments that could enhance nephelometry's utility.

    Main Methods:

    • Review of light scattering theory for small spheres.
    • Analysis of commercially available nephelometers for immunoassays.

    Related Experiment Videos

  • Discussion of factors affecting assay sensitivity and precision.

    • Main Results:

    • Nephelometry's theoretical ability to determine particle characteristics.
    • Identified limitations include sensitivity to noise from dust and aggregates.
    • Current commercial nephelometers utilize diverse light sources and often include microcomputers.

    Conclusions:

    • Nephelometry is a valuable technique for immunoassays due to its simplicity and precision.
    • Noise sensitivity remains a key challenge for widespread application.
    • Advancements in light scattering theory and instrumentation may broaden nephelometry's role in future immunoassays.