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Related Concept Videos

Gastrulation01:56

Gastrulation

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Neurulation01:30

Neurulation

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Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
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Cleavage and Blastulation01:33

Cleavage and Blastulation

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After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
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Embryonic Connective Tissues01:20

Embryonic Connective Tissues

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During early development, the embryo forms two types of connective tissues— the mesenchyme and mucoid connective tissue.
The mesenchyme is the first connective tissue that emerges in the developing embryo. It consists of loosely arranged multipotent mesenchymal cells and reticular fibers in the extracellular matrix. This loose arrangement allows easy migration of cells, which is essential for germ layer positioning, patterning, and organ morphogenesis during embryonic development.
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Related Experiment Video

Updated: Jul 27, 2025

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
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Rosettes guide the way for mesodermal MET.

Hannah R Moran1, Christian Mosimann1

  • 1Section of Developmental Biology, Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

Developmental Cell
|June 6, 2023
PubMed
Summary
This summary is machine-generated.

Multicellular rosettes are key to mesenchymal-epithelial transition (MET) during early development. These structures guide tissue morphogenesis in notochord and lateral plate mesoderm formation.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Morphogenesis

Background:

  • Mesenchymal-epithelial transition (MET) is crucial for embryonic development.
  • MET drives tissue morphogenesis and cell fate determination.
  • Understanding MET's regulation is vital for developmental biology.

Purpose of the Study:

  • To investigate the role of multicellular rosettes in MET.
  • To elucidate the contribution of rosettes to notochord and lateral plate mesoderm formation.
  • To reveal novel mechanisms regulating early embryonic development.

Main Methods:

  • Utilized advanced imaging techniques.
  • Employed genetic manipulation in model organisms.
  • Analyzed cellular behaviors during rosette formation and MET.

Main Results:

  • Multicellular rosettes were identified as critical structures facilitating MET.
  • Rosettes play a significant role in the formation of notochord and lateral plate mesoderm.
  • Specific cellular dynamics within rosettes were found to drive epithelialization.

Conclusions:

  • Multicellular rosettes are essential for MET in early embryonic development.
  • These findings provide new insights into tissue morphogenesis.
  • The study highlights a conserved mechanism for epithelialization.