Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

5.4K
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
5.4K
Role of Vitamins in Maintaining Bone Health01:25

Role of Vitamins in Maintaining Bone Health

3.5K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.5K
Vitamins01:30

Vitamins

826
Vitamins, derived from the Latin word for life, are essential organic substances required in small quantities for optimal growth and overall well-being. Unlike other organic nutrients, vitamins don't act as sources of energy or building materials but rather facilitate these nutrients' utilization by the body. Vitamins are predominantly coenzymes, assisting enzymes in specific chemical actions, like the oxidation of glucose for energy involving B vitamins. Most vitamins are not produced...
826
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

2.8K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.8K
Skeleton and Calcium Homeostasis01:21

Skeleton and Calcium Homeostasis

4.6K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.6K
Connective Tissue Cell Types01:22

Connective Tissue Cell Types

3.3K
Connective tissue develops from the mesoderm of a developing embryo and consists of cells, fibers, and ground substance: a gel-like material containing large complexes of carbohydrates and proteins. Connective tissue was first identified as a separate tissue family in the 18th century, and Johannes Peter Muller coined the term connective tissue.
Fat cells (adipocytes), smooth muscle cells (myoblasts), and bone cells (osteoblasts) are some connective tissue cell types. Some immune system cells...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EFFECTS OF CHEMICAL REAGENTS ON THE CYCLIC CHANGES OF CORTEX AND CYTOPLASM IN THE ACTIVATED ENUCLEATED EGG-FRAGMENTS OF THE SEA URCHIN.

Development, growth & differentiation·2023
Same author

Acceleration of the Cleavage in Sea Urchin Eggs by Treatmentswith Local Anesthetics (I) Procaine Treatment*: (acceleration of cleavage/local anesthetics/procaine/sea urchin eggs).

Development, growth & differentiation·2023
Same author

RELATIONSHIPS BETWEEN DISTRIBUTION OF TUBULIN-PARACRYSTALS, GRANULES STAINING WITH NEUTRAL RED AND CLEAVAGE ACTIVITY IN SEA URCHIN EGG-FRAGMENTS

Development, growth & differentiation·2023
Same author

Acceleration of the Cleavage in Sea Urchin Eggs by Treatments with Local Anesthetics (II) Treatments with Some Other Local Anesthetics than Procaine*: (acceleration of cleavage/local anesthetics/caffeine/sea urchin eggs).

Development, growth & differentiation·2023

Related Experiment Video

Updated: Jul 27, 2025

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data
10:46

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data

Published on: December 9, 2015

10.7K

Effects of D

Manabu K Kojima1

  • 1Sugashima Marine Biological Laboratory, Nagoya University, Sugashima-cho, Toba-shi, Mie-ken 517, Japan.

Development, Growth & Differentiation
|June 7, 2023
PubMed
Summary

Deuterium oxide (D2O) can induce parthenogenetic development in sea urchin eggs, leading to development up to the gastrula stage. Combined treatments with D2O enhance this effect, even activating enucleated egg halves.

Area of Science:

  • * Developmental Biology
  • * Marine Biology
  • * Cell Biology

Background:

  • * Unfertilized sea urchin eggs typically require sperm for activation and development.
  • * Previous studies indicated limited developmental capacity of D2O-treated eggs.
  • * Sea urchin eggs serve as a model system for studying early embryonic development and activation.

Purpose of the Study:

  • * To investigate the potential of deuterium oxide (D2O) in inducing parthenogenetic development in sea urchin eggs.
  • * To compare the effectiveness of single and combined D2O treatments for artificial parthenogenesis.
  • * To determine if D2O can activate enucleated sea urchin egg fragments.

Main Methods:

  • * Treatment of unfertilized sea urchin eggs with deuterium oxide (D2O)-enriched seawater.

More Related Videos

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.4K
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

5.5K

Related Experiment Videos

Last Updated: Jul 27, 2025

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data
10:46

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data

Published on: December 9, 2015

10.7K
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.4K
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

5.5K
  • * Application of combined treatments, including D2O with hypertonic seawater or butyric acid.
  • * Activation of centrifugally enucleated egg halves using D2O treatments.
  • * Observation and assessment of embryonic development stages.

    • Main Results:

    • * D2O treatment induced fertilization membrane elevation, cytaster formation, and cleavage in unfertilized sea urchin eggs.
    • * D2O-treated eggs developed to the gastrula stage, contradicting previous reports.
    • * Combined D2O treatments (e.g., D2O + hypertonic seawater, D2O + butyric acid) were more effective than single D2O treatment.
    • * Centrifugally enucleated egg halves activated by D2O developed to a blastulalike stage.

    Conclusions:

    • * Deuterium oxide (D2O) is a potent agent for inducing artificial parthenogenesis in sea urchin eggs.
    • * Combined D2O treatments significantly enhance the induction of parthenogenetic development.
    • * D2O can activate enucleated egg fragments, demonstrating its role in initiating developmental processes.