Related Experiment Video

Updated: Jul 27, 2025

In Ovo Intravascular Injection in Chicken Embryos
07:00

In Ovo Intravascular Injection in Chicken Embryos

Published on: June 3, 2022

6.1K

Appearance of Primordial Germ Cells in Young Chick Blastoderms Cultured in vitro: (primordial germ

L A Sutasurya1, S Yasugi1, T Mizuno1

  • 1Zoological Institute, Faculty of Science, University of Tokyo, Hongo, Tokyo 113.

Development, Growth & Differentiation
|June 7, 2023
PubMed

Insights

Primordial germ cells (PGCs) in chick embryos originate in the epiblast before primitive streak formation. The underlying hypoblast layer is not essential for PGC differentiation.

Area of Science:

  • Developmental biology
  • Embryology
  • Cell biology

Background:

  • Primordial germ cells (PGCs) are crucial for sexual reproduction.
  • Understanding PGC origins and migration is fundamental to developmental biology.

Purpose of the Study:

  • To investigate the appearance and location of PGCs in early chick embryos.
  • To determine the role of the epiblast and hypoblast layers in PGC development.

Main Methods:

  • Cultivation of isolated epiblast and hypoblast layers from young chick blastoderms.
  • Observation of presumptive PGCs' location and translocation during early embryonic development.

Main Results:

  • Presumptive PGCs are present in the epiblast before primitive streak formation.
  • PGCs gradually translocate to the lower layer during primitive streak formation, with many remaining in the upper layer.
  • The primary hypoblast is dispensable for PGC differentiation.

Conclusions:

  • PGCs originate in the epiblast layer of chick embryos.
  • Hypoblast is not required for the differentiation of PGCs, suggesting epiblast autonomy in early PGC development.

More Related Videos

Method for Culture of Early Chick Embryos ex vivo New Culture08:45

Method for Culture of Early Chick Embryos ex vivo New Culture

Published on: October 20, 2008

15.8K
A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos07:29

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Published on: December 28, 2016

22.3K

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
45.4K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
5.3K
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
57.7K