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Updated: Jul 27, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
TRANSCRIPTIONAL CONTROL OF PROTEIN SYNTHISIS IN THE DEVELOPING OVARIES OF BOMBYX MORI
Masami Yamao1, Hideki Shimizu1, Osamu Nakamura1
1Agricultural Chemistry Institute, Kyushu University, Fukuoka 812, Japan.
Abstract:
Amino acid incorporation was studied with cell-free extracts and ribosomes prepared from pupal ovaries at different ages of Bombyx mori. Poly(U)-directed 3 H-phenylalanine incorporation attained a maximum rate at a certain stage of development, but soon dropped to a low level and was replaced by 3 H-leucine incorporation, which was due to endogenous mRNA. The latter incorporation occurred at the stage when actual protein synthesis takes place in the ovaries. "Run-off" of the ribosomes which had a high endogenous activity resulted in an enhancement of the poly(U)-dependent activity. The results indicate that the protein synthesis in the ovary is mainly controlled at the level of mRNA. This was further supported by the fact that the relative amount of an ovarian poly(A)-containing "mRNA" fraction increased in parallel with the endogenous activity.
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