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Author Spotlight: Analyzing the Synaptic Ultrastructure in Mature Retinal Organoids Using TEM

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ULTRASTRUCTURAL STUDY OF THE PROCESS OF ROSETTE FORMATION BY DISSOCIATED NEURAL RETINAL CELLS IN STATIONARY CULTURE.

Hajime Fujisawa¹

¹Department of Anatomy, Kyoto Prefectural University of Medicine, Kamikyo-ku, Kyoto 602, Japan.

Development, Growth & Differentiation

|June 7, 2023

Summary

Chick embryo neural retinal cells form rosettes through cell adhesion. Mitochondria-rich cell portions establish zonula adhaerens junctions, guiding radial cell arrangement in developing rosettes.

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Last Updated: Jul 27, 2025

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Published on: June 7, 2024

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Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Published on: December 23, 2012

Area of Science:

Developmental biology
Cell biology
Neuroscience

Background:

Neural retinal cells exhibit distinct morphological polarity.
Understanding cell-cell adhesion mechanisms is crucial for tissue development.

Purpose of the Study:

To investigate the process of rosette formation in dissociated chick embryo neural retinal cells.
To identify the specific cell junctions involved in rosette morphogenesis.

Main Methods:

Stationary cultures of dissociated neural retinal cells from 5 1/2-day-old chick embryos.
Electron microscopy to observe cell aggregates during rosette formation.

Main Results:

Dissociated neural retinal cells possess clear morphological polarity.
Initial cell-to-cell adhesion occurs randomly.
Zonula adhaerens junctions form between mitochondria-containing portions of adjacent cells.
Increasing cell contact area promotes radial arrangement and rosette formation.

Conclusions:

Rosette formation in neural retinal cell cultures is a stepwise process driven by cell polarity and specific junction formation.
Zonula adhaerens junctions play a key role in establishing the radial organization of cells within rosettes.