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Updated: Jul 27, 2025
Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
Published on: June 9, 2012
MOUSE MUSCLE CELL DIFFERENTIATION IN CLONAL CULTURE
Harukazu Nakamura1, Hajime Fujisawa1, Kenji Watanabe1
1Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyoku, Kyoto 602, Japan.
Abstract:
Clonal culture of muscle cells from thigh muscles of the term fetus of the mouse was undertaken. Myoblasts were spherical or spindle-shaped, and proliferated exponentially until day 4 in culture. The generation time of the muscle cells from 2 to 4 days' culture was 9.1 to 13.4 hr. The fusion of myoblasts began on day 4 in culture; many myotubes had been formed by day 6 and spontaneous contraction was observed on day 7. Clonal efficiency was 30%, and the proportion of muscle colonies in all the colonies was 72%.
