Related Experiment Video
Updated: Jul 27, 2025
Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
Published on: November 17, 2016
The Inhibitory Effect of 5-Bromodeoxyuridine on the Programmed Cell Death in the Chick Limb: (cell death/BrdU/limb
Shigenobu Toné1, Shoji Tanaka1, Yoshihiro Kato1
1Department of Developmental Biology, Mitsubishi-Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194, Japan.
Abstract:
The inhibitory effect of 5-bromodeoxyuridine (BrdU) on the programmed cell death in interdigital mesenchyme of a chick leg bud was studied using vital staining and histological methods. A single administration of BrdU at day 6 1/3 specifically inhibited the programmed cell death in the mesenchyme of interdigital areas of a leg bud, resulting in the formation of a web-like structure. No inhibitory effect was observed on general development of limb buds. Hoechst 33258 staining revealed that many cells of interdigits incorporated BrdU into their nuclei. The simultaneous administration of thymidine blocked the BrdU effect.
More Related Videos
06:49Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
Published on: January 12, 2022
06:44Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Published on: April 28, 2021
