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Updated: Jul 27, 2025
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High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 21, 2014
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INITIATION OF CLEAVAGE IN FISH EGGS BY INJECTION OF FLAGELLA OR MICROTUBULES OF SEA URCHIN SPERMATOZOA
Tadayuki Ohta1, Takashi Iwamatsu1
1Biological Laboratory, Aichi University of Education, Igaya-cho, Kariya-shi, Japan 448.
Development, Growth & Differentiation
|June 7, 2023
Abstract:
Flagella and their microtubules obtained from sea urchin (Hemicentrotus pulcherrimus) spermatozoa were injected into unfertilized eggs of the medaka (Oryzias latipes) with a micropipette. Upon activation, some of the eggs began the first cleavage with three or more irregular blastomeres, and developed to the morula stage. It is suggested that sperm flagellar microstubule material is one of the cleavage initiation substances.
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