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HORMONAL CONTROL OF OOCYTE MATURATION IN ARENICOLA MARINA L. (ANNELIDA, POLYCHAETA) II. MATURATION AND FERTILIZATION.

L Meijer1

  • 1Université des Sciences el Techniques de Lille I, Service de Biologie Animale, Laboratoire ďEndocrinologie Comparée des Invertébrés (Prof. M. DURCHON) (L. A. CNRS No. 148), B P. 36. 59650-Villeneuve ďAscq, FRANCE.

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Summary

Oocyte maturation in Arenicola marina is hormonally controlled, enabling fertilization and parthenogenetic activation. This transition from prophase to metaphase arrest is crucial for reproductive competence.

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Area of Science:

  • Marine biology
  • Developmental biology
  • Reproductive biology

Background:

  • Oocytes of Arenicola marina are arrested in meiotic prophase until spawning.
  • Hormonal regulation controls oocyte maturation, advancing them to metaphase arrest.

Purpose of the Study:

  • To investigate the hormonal control of oocyte maturation in Arenicola marina.
  • To determine the stage-specific fertilizability and parthenogenetic activation of oocytes.
  • To examine sperm-oocyte interactions during fertilization.

Main Methods:

  • In vitro incubation of oocytes with brain extract.
  • Assessment of fertilization and parthenogenetic activation using ionophore A23187 and glycerol.
  • Scanning electron microscopy (SEM) of sperm-oocyte interactions.

Main Results:

  • Prophase-arrested oocytes are unfertilizable and unactivatable.
  • Metaphase-arrested oocytes become fertilizable and parthenogenetically activatable.
  • Fertilizability correlates with microvilli retraction during maturation.
  • SEM revealed early sperm contact and penetration dynamics.

Conclusions:

  • Hormonal maturation is essential for Arenicola marina oocyte reproductive competence.
  • Fertilizability is acquired during meiotic maturation, linked to morphological changes.
  • Sperm behavior and interaction with the oocyte surface are critical for fertilization.