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Updated: Jul 27, 2025

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
HORMONAL CONTROL OF OOCYTE MATURATION IN ARENICOLA MARINA L. (ANNELIDA, POLYCHAETA) II. MATURATION AND FERTILIZATION
1Université des Sciences el Techniques de Lille I, Service de Biologie Animale, Laboratoire ďEndocrinologie Comparée des Invertébrés (Prof. M. DURCHON) (L. A. CNRS No. 148), B P. 36. 59650-Villeneuve ďAscq, FRANCE.
Abstract:
In Arenicola marina (Annelida, Polychacta) the oocytes arc arrested in the first prophase stage of meiosis until spawning. Oocyte maturation is under hormonal control: when incubated in vitro in a brain extract oocytes proceed to the first metaphase at which they remain arrested until fertilization. The prophase arrested oocytes can neither be fertilized nor parthcnogenetically activated by ionophore A23187 or 1 M glycerol. On the contrary the metaphase-arrested oocytes can be fertilized and parthenogenetically activated. Fertilizability thus appears during maturation; it seems to be linked to microvilli retraction. A study of spermatozoa "capacitation" and oocyte fertilization or activation is reported. A scanning electron microscope study of early contact and penetration of spermatozoa is presented.
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