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Updated: Jul 27, 2025

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
HORMONAL CONTROL OF OOCYTE MATURATION IN ARENICOLA MARINA L. (ANNELIDA, POLYCHAETA) I. MORPHOLOGICAL STUDY OF OOCYTE
1Université des Sciences et Techniques de Lille I, Service de Biologie Animate, Laboratoire ď d'Endocrinologie Comparée des Invertébrés (Prof. M. DURCHON) (L. A. CNRS No. 148), B. P. 36, 59650-Villeneuve ď Ascq, FRANCE.
Abstract:
In Arenicola marina (Annelida, Polychaeta) the oocytes are arrested in the first prophase stage of mciosis until spawning. Oocyte maturation is under hormonal control: when incubated in vitro in a brain extract oocytes reach the first metaphase at which they remain arrested until fertilization. The meiosis reinitiating substance induces numerous morphological changes in the oocytes: general (shape), cortical (microvilli retraction, plasma membrane flattening), cytoplasmic (cortical granules repartition) and nuclear modifications (germinal vesicle breakdown, chromosome condensation, formation of a meiotic maturation spindle). A kinetic study of these morphological modifications has been performed.
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