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Published on: November 17, 2016
Distribution of Retinoids Applied Exogenously to Chick Limb Buds: an Autoradiographic Analysis: (Retinoic Acid/Limb
Koji Tamura1, Yuichi Hashimoto2, Koichi Shudo3
1Biological Institute, Tohoku University, Sendai 980.
Abstract:
An autoradiographic analysis was undertaken to examine the localization of retinoids applied exogenously to chick limb buds. Ion-exchange beads (AGI-X2) containing a tritium-labeled synthetic retinoid, Am80, were implanted to various regions of chick wing buds. This synthetic retinoid is known to induce a duplicated limb pattern as retinoic acid (RA) does. One to 24 hours after the application, wing buds were fixed, sectioned, and prepared for autoradiography. Heavy labeling was observed in the peripheral region of the wing mesoderm, but no gradient along the antero-posterior axis was found. These results suggest that the peripheral region of the limb bud may be important for the morphogenetic function of RA. Tissue-bound retinoids may not form an antero-posterior concentration gradient when retinoids are added to the anterior margin of the chick limb bud.
