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A Method for Microinjection of Patiria minata Zygotes
Published on: September 1, 2014
MEMBRANE REACTIONS DURING FERTILIZATION AND 1-METHYLADENINE STIMULATION IN IMMATURE STARFISH OOCYTES
Harold H Lee1, Ellen Goll1, Mary Plenzler1
1Department of Biology, The University of Toledo, Toledo, Ohio 43606, U. S. A.
Abstract:
Immature oocytes from starfishes were obtained during the breeding and non-breeding seasons. The oocytes were "fertilized" without previous induction of maturation by 1-methyladenine (1-MA) or germinal vesicle breakdown (GVB). Elevation of the fertilization membrane (FM) was observed with eggs obtained during the breeding season, but not observed with eggs obtained during the non-breeding season. Abnormal development was observed when these "fertilized" eggs with a FM were subsequently treated with 1-MA. The results of these experiments indicated that the elevation of FM was independent of GVB or the mixing of nuclear and cytoplasmic components.
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