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Updated: Jul 27, 2025
High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 21, 2014
INHIBITION OF DIHYDROFOLATE REDUCTASE BY PALMITOYL-CoA AND THE REVERSAL OF THE INHIBITION BY SPERMINE AND SPERMIDINE
Shinichiro Kusunoki1, Ikuo Yasumasu1
1Department of Biology, School of Education, Waseda University, 1-6-1, Nishiwaseda, Shinjuku-ku, Tokyo, 160, Japan.
Abstract:
Dihydrofolate reductase activity in fertilized eggs of the sea urchin, Hemicentrotus pulcherrimus, was almost the same as in unfertilized eggs. Aminopterin inhibited the enzyme competitively with dihydrofolate (FH2 ). The apparent Km value for FH2 in the dihydrofolate reductase reaction was about 0.1 μM in the crude homogenate of both unfertilized and fertilized eggs. Dihydrofolate reductase in the eggs was also inhibited by palmitoyl-CoA. The inhibition was canceled by polyamines, especially by spermine, but putrescine failed to prevent the enzyme from the inhibition. The change in long-chain acyl-CoA and polyamine concentrations during fertilization are discussed as possible regulatory factors of the enzyme.
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