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Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

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Published on: January 12, 2015

STUDIES ON THE GASTRULATION OF AMPHIBIAN EMBRYOS: NUMERICAL CRITERIA FOR STAGE DETERMINATION DURING BLASTULATION AND

Norio Nakatsuji¹

¹Department of Zoology, Faculty of Science, University of Kyoto, Sakyo-ku, Kyoto 606, Japan.

Development, Growth & Differentiation

|June 7, 2023

Abstract:

The changing external features of Xenopus laevis embryos were examined from the first cleavage through the late gastrula as a means to develop quantitative criteria for the determination of stages for this period. During blastulation, the cell number along arcs centered on the animal or vegetal pole and representing one-sixth of a meridian - called the MCNo. - can be used as such a criterion. During gastrulation, the width of the blastopore divided by the diameter of the whole embryo can be used as such a criterion.

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