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PURIFICATION AND PROPERTIES OF THE PROTHORACICOTROPIC HORMONE OF THE SILKWORM, BOMBYX MORI
Hiromichi Nagasawa1, Akira Isogai1, Akinori Suzuki1
1Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
Abstract:
A simple and highly reproducible procedure for partial purification of prothoracicotropic hormone (PTTH) was established starting with 96,000 male adult Bombyx heads. Approximately 28,500-fold purification of PTTH was accomplished with a yield of about 50% and 6 ng of the most purified preparation ("highly purified PTTH") caused adult development in a brainless Samia pupa. The peptidal nature of PTTH was reconfirmed through the effects of various enzymatic and chemical treatments on the biological activity of "highly purified PTTH". Gel-filtration indicated the molecular weight of PTTH to be 4,400.
