Mitochondrial Protein Synthesis During Embryonic Development of Cricket: (cricket embryo/mitochondria/protein
Satoshi Asada1, Chiyuki Tateishi1, Tohru Nakazawa1
1Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, 274 Japan.
Abstract:
Protein synthetic activity in homogenate of embryos of cricket, Gryllus bimaculatus, increased transiently 25 hr after oviposition, though the activity in mitochondrial fraction increased successively thereafter. The decrease in the activity after the peak in homogenate was caused apparently by increase in the amino acid content in cytosol during development. To analyze increase in the mitochondrial protein synthetic activity 25 hr after oviposition, effect of various components was examined in the reconstituted mitochondrial system. The nucleic acids extracted from 25-hr embryos most effectively stimulated the protein synthetic activity, but those from 0-hr embryos did not. The results were discussed on the role of mitochondrial protein synthesis during development of cricket embryos.
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