Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stromal Transplantation and corneal-sparing techniques in ectatic diseases.

Progress in retinal and eye research·2026
Same author

Bag-in-the-lens technique.

Frontiers in ophthalmology·2026
Same author

Driving Forces and Perceived Barriers for the Adoption of Novel Endothelial Cell Therapies: An International Survey of Corneal Surgeons.

Cornea·2026
Same author

Machine Learning for Predicting Coronary Heart Disease Risk in Patients with Hypertension: An Ensemble Modeling Approach.

Healthcare informatics research·2026
Same author

Enhancing patient education in cataract surgery using a conversational artificial intelligence chatbot: pilot randomized controlled trial.

Journal of cataract and refractive surgery·2025
Same author

Influence of Topical Fluorescein on Scheimpflug-Based Corneal Densitometry and Potential Applications.

Cornea·2025

Related Experiment Video

Updated: Jul 27, 2025

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
10:46

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Published on: September 17, 2014

15.3K

17 Cell viability after DMEK preparation.

Anita Sajet1,2, Alina Miron2, Esther Groeneveld-van Beek1,2

  • 1Netherlands Institute for Innovative Ocular Surgery, Rotterdam, Netherlands.

BMJ Open Ophthalmology
|June 7, 2023
PubMed
Summary

Graft preparation and storage minimally impact endothelial cell viability for Descemet membrane endothelial keratoplasty (DMEK) grafts. An additional cell density check post-preparation can reduce complications.

More Related Videos

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
11:57

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Published on: March 6, 2019

10.4K
An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

6.7K

Related Experiment Videos

Last Updated: Jul 27, 2025

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
10:46

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Published on: September 17, 2014

15.3K
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
11:57

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Published on: March 6, 2019

10.4K
An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

6.7K

Area of Science:

  • Ophthalmology
  • Transplantation Biology
  • Corneal Surgery

Background:

  • Descemet membrane endothelial keratoplasty (DMEK) is a surgical procedure for corneal blindness.
  • Graft preparation and storage are critical steps influencing outcomes.
  • Endothelial cell density (ECD) and viability are key determinants of DMEK graft success.

Purpose of the Study:

  • To assess the impact of graft preparation and organ-culture storage on ECD and viability of DMEK grafts.
  • To determine if these processes affect endothelial cell health before transplantation.

Main Methods:

  • DMEK grafts (n=27) were prepared and evaluated for cell viability (Calcein-AM staining) and ECD.
  • Evaluations included light microscopy (LM ECD) and Calcein-ECD.
  • Grafts were assessed directly post-preparation and after 3-7 days of storage.

Main Results:

  • LM evaluation showed an intact endothelial monolayer immediately after preparation.
  • Calcein-ECD was 18% lower than LM ECD for 5 initially allocated grafts.
  • Paired grafts showed minimal median ECD decrease (1% post-preparation, 2% after storage).
  • Viable cell coverage remained high (88% post-preparation, 92% after storage).

Conclusions:

  • Graft preparation and storage generally preserve endothelial cell viability.
  • Some grafts may exhibit early endothelial cell damage, but storage causes minimal additional changes.
  • Implementing pre-transplant ECD evaluation in eye banks may improve DMEK outcomes.