Therapeutic effect of menstrual blood stem cells in rats with thin endometrium

To explore the therapeutic effect of transplantation of menstrual blood stem cells (MenSCs) in rats with thin endometrium.

Thirty SPF grade female SD rats aged 8-10 weeks were randomly divided into model control group and MenSC group, with 15 rats in each group. The thin endometrium injury model was prepared by chemical method in one side of the uterus of both groups. On the 7th day of modeling, normal saline or the third generation of MenSCs were injected into the model uterus at multiple points, and the other side of the uterus was used as an internal control without treatment. HE staining was used to observe the histological structure of endometrium; immunohistochemical staining was used to observe the expression of cyto-keratin (CK) 18 and vimentin in endometrial tissue; 5-ethynyl-2'-deoxyuridine (EdU) cell proliferation assay was used to evaluate the cell proliferation in endometrial tissue; immunofluorescence staining was used to detect the expression of vascular endothelial marker CD34 and vascular endothelial growth factor (VEGF) in endometrial tissue; the expression of leukemia inhibitory factor (LIF), integrin β3 (ITGβ3) and homeobox A10 (HOXA10) in endometrial tissue was determined by realtime RT-PCR. After treatments, the female and male rats were caged in a ratio of 2∶1 to observe the effect of MenSC on the reproductive function of thin endometrium model rats.

Compared with the surgical control group, the endometrium in the model control group was thinner, and the numbers of glands and blood vessels were less (all P<0.01). After MenSC transplantation, the thickness of endometrium, the numbers of blood vessels and glands were significantly increased (all P<0.01). The proliferative cells in the basal layer of endometrium in MenSC group were more than those in the model control group (P<0.05), and the expression of vimentin, CK18, CD34 and VEGF in the uterus of rats in MenSC group were significantly higher than those in the model control group (P<0.05). LIF, ITGβ3 and HOXA10 gene expression levels were also significantly higher than those in the model control group (all P<0.05). The results of pregnancy experiment showed that the number of embryo implantation in MenSC group was higher than that in model control group (P<0.05).

MenSC transplantation can promote the proliferation of endometrial cells, upregulate vimentin, CK18, CD34 and VEGF levels, and promote the recovery of endometrial morphology and function, thus improving the endometrial receptivity and fertility of the rats with thin endometrium.