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Related Experiment Video

Updated: Jul 27, 2025

A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
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New seed coating containing

Sena Turkan1,2, Agnieszka Mierek-Adamska1,2, Milena Kulasek1,2

  • 1Department of Genetics/Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Toruń, Poland.

Peerj
|June 7, 2023
PubMed
Summary
This summary is machine-generated.

A novel seed coating using Trichoderma viride enhances canola growth and protects against fungal pathogens. This eco-friendly approach offers a scalable solution for sustainable agriculture and food security.

Keywords:
Brassica napus L.GerminationPlant growth-promoting fungi (PGPF)Plant stringent responseRelA/SpoT homologsSeed coatingTrichoderma

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Area of Science:

  • Agricultural Science
  • Plant Pathology
  • Biotechnology

Background:

  • Plant growth-promoting fungi (PGPF) like Trichoderma can improve crop yield and stress tolerance, reducing agrochemical needs.
  • Large-scale PGPF application is limited by delivery system constraints.
  • Seed coating is an emerging, efficient method for delivering PGPF.

Purpose of the Study:

  • To develop and evaluate a new seed coating containing Trichoderma viride for canola (Brassica napus L.).
  • To assess the antifungal efficacy of the seed coating against key canola pathogens.
  • To determine the impact of the seed coating on canola germination, seedling growth, and stress response.

Main Methods:

  • A seed coating was formulated using chitin, methylcellulose, and Trichoderma viride spores.
  • Antifungal activity of T. viride was tested against Botrytis cinerea, Fusarium culmorum, and Colletotrichum sp.
  • Germination ratio, seedling growth, superoxide dismutase (SOD) activity, and RSH gene expression were analyzed.

Main Results:

  • T. viride significantly inhibited the growth of all tested canola pathogens, with over 40% inhibition for F. culmorum.
  • The seed coating did not adversely affect seed germination.
  • Seedling growth was enhanced, and no plant stress response was induced by the seed coating.

Conclusions:

  • A cost-effective, environmentally responsible seed coating for T. viride was successfully developed.
  • The seed coating is suitable for industrial-scale application.
  • This innovation supports sustainable agriculture by promoting plant health and yield.