A high-performance electrochemical biosensor using an engineered urate oxidase
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Updated: Jul 27, 2025

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Electrochemical Preparation of Poly3,4-Ethylenedioxythiophene Layers on Gold Microelectrodes for Uric Acid-Sensing Applications
Published on: July 28, 2021
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Zheng Wei1, Tanja Knaus1, Yuxin Liu1
1Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands. f.mutti@uva.nl.
Abstract:
We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM-1 mM), and more than 28 days lifetime.

