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Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
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Vacuolar Ca

Andrew M Beacham1, Katie A Wilkins2, Julia M Davies2

  • 1Centre for Crop and Environmental Sciences, Harper Adams University, Edgmond, Shropshire, TF10 8NB, UK.

Plant Physiology and Biochemistry : PPB
|June 7, 2023
PubMed
Summary
This summary is machine-generated.

Lettuce tipburn, a physiological disorder, is linked to calcium transport. Differential expression of vacuolar calcium transporters in resistant lettuce cultivars suggests their importance for breeding improved varieties.

Keywords:
CalciumLactuca sativaLettuceTipburn

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Area of Science:

  • Plant physiology
  • Molecular genetics
  • Agricultural science

Background:

  • Tipburn is a significant physiological disorder in lettuce (Lactuca sativa), causing leaf discoloration and quality issues in the fresh produce industry.
  • The underlying physiological and molecular basis of tipburn is poorly understood, though it is associated with nutrient deficiencies, particularly calcium.
  • Vacuolar calcium transporters play a role in calcium homeostasis and exhibit differential expression in tipburn-resistant and susceptible *Brassica oleracea*.

Purpose of the Study:

  • To investigate the expression of *L. sativa* vacuolar calcium transporter homologues in tipburn-resistant and susceptible lettuce cultivars.
  • To identify specific *L. sativa* vacuolar calcium transporter genes associated with tipburn resistance.
  • To understand the role of differential gene expression in the development of tipburn resistance in lettuce.

Main Methods:

  • Analysis of gene expression of *L. sativa* vacuolar calcium transporter homologues, including Ca2+/H+ exchangers and Ca2+-ATPases.
  • Comparison of gene expression levels between tipburn-resistant and susceptible lettuce cultivars.
  • Examination of gene expression in both symptomatic and asymptomatic leaves of susceptible cultivars.

Main Results:

  • Some *L. sativa* vacuolar calcium transporter homologues showed higher expression in resistant cultivars, while others were higher in susceptible cultivars or unaffected by tipburn phenotype.
  • Certain homologues were more highly expressed in symptomatic leaves of susceptible cultivars, indicating that induced expression does not confer resistance.
  • Differential baseline expression of vacuolar calcium transporter genes appears crucial for tipburn resistance in lettuce.

Conclusions:

  • Specific vacuolar calcium transporter genes are associated with tipburn resistance in lettuce.
  • Understanding the baseline expression of these genes can aid in breeding for tipburn resistance.
  • This knowledge can contribute to the development of more resilient lettuce varieties, improving crop quality and reducing industry losses.