Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
Homologous Recombination02:31

Homologous Recombination

62.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Long non-coding RNAs and exosomal lncRNAs: Potential functions in lung cancer progression, drug resistance and tumor microenvironment remodeling" [Biomed. Pharmacother. 150 (2022) 112963].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Retraction notice to "Green products from herbal medicine wastes by subcritical water treatment" [J Hazard Mater 424 (2022) 127294].

Journal of hazardous materials·2026
Same author

SERS-Enhanced CRISPR Biosensors: A Platform for Ultrasensitive Molecular Diagnostics.

Analytical chemistry·2026
Same author

Bio-Inspired Smart Elastin-Like Polypeptides (ELPs) for Precision Drug Delivery: Molecular Strategies, Thermal Responsiveness, and Translational Advances.

Advanced healthcare materials·2026
Same author

Hemoglobin as a Molecular Glue: Toward Potent Inhibition of HbS Polymerization in Sickle Cell Disease.

Advanced healthcare materials·2026
Same author

Retraction notice to "Highly antifouling polymer-nanoparticle-nanoparticle/polymer hybrid membranes" [Sci. Total Environ. 810 (2022) / 152228].

The Science of the total environment·2026
Same journal

Feedstock-dependent oxidation and immobilization of As(III) by iron-modified biochars: The role of intrinsic lignin in enhancing electron transfer.

Environmental research·2026
Same journal

MOFs mixed matrix membranes for CO<sub>2</sub> separation: material design, optimization strategies, and industrial pathways.

Environmental research·2026
Same journal

The fungicide thiram alters the embryo-larval development of the black sea urchin Arbacia lixula by inducing multifactorial toxicological responses.

Environmental research·2026
Same journal

Sulfur vacancy-mediated selective S-O bond scission dictates dominant singlet oxygen evolution in PMS activation.

Environmental research·2026
Same journal

Prenatal exposure to particulate matter and risk of autism spectrum disorder in children: a population-based cohort study in South Korea.

Environmental research·2026
Same journal

Graphene Quantum Dots-Bridged Fe-Ce Bimetallic Aerogel Catalyst for Efficient Alkaline Fenton Removal of Three Nitrogen Species (NH<sub>3</sub>-N, NO<sub>3</sub><sup>-</sup>-N, and NO<sub>2</sub><sup>-</sup>-N).

Environmental research·2026
See all related articles

Related Experiment Video

Updated: Jan 18, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

28.0K

Natural components as surface engineering agents for CRISPR delivery.

Navid Rabiee1

  • 1Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth, WA, 6150, Australia; School of Engineering, Macquarie University, Sydney, NSW, 2109, Australia.

Environmental Research
|June 7, 2023
PubMed
Summary
This summary is machine-generated.

Natural components offer eco-friendly surface engineering for CRISPR delivery, enhancing efficiency and overcoming traditional method limitations. This approach promises biocompatible, biodegradable, and cost-effective solutions for gene editing applications.

Keywords:
CRISPRDelivery systemsNatural componentsSurface engineering

More Related Videos

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.1K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.5K

Related Experiment Videos

Last Updated: Jan 18, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

28.0K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.1K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.5K

Area of Science:

  • Biotechnology
  • Materials Science
  • Nanotechnology

Background:

  • CRISPR delivery faces challenges with traditional methods, including safety concerns and limitations.
  • Surface engineering of delivery vehicles presents a promising strategy to enhance CRISPR system performance.
  • Natural and environmentally friendly components are emerging as viable alternatives for surface modification.

Purpose of the Study:

  • To explore the potential of natural and environmentally friendly components as surface engineering agents for CRISPR delivery.
  • To provide an overview of current research on utilizing natural materials for nanoparticle and nanomaterial surface modification.
  • To discuss the advantages, challenges, and future perspectives of natural component-based CRISPR delivery systems.

Main Methods:

  • Review of current literature on surface engineering for CRISPR delivery.
  • Analysis of natural components such as lipids, proteins, leaf extracts, and polysaccharides for surface modification.
  • Discussion of novel inorganic nanomaterials (MOF, MXene) and their synergistic potential with natural components.

Main Results:

  • Natural components offer advantages like biocompatibility, biodegradability, engineered functionality, cost-effectiveness, and environmental friendliness.
  • Surface modification with natural components can improve delivery efficiency, stability, and cellular internalization of CRISPR systems.
  • Novel nanomaterials like MOF and MXene show potential for enhanced CRISPR delivery when combined with natural components.

Conclusions:

  • Natural components as surface engineering agents can overcome limitations of traditional CRISPR delivery methods.
  • This approach offers a promising, eco-friendly, and biocompatible strategy for advancing gene editing technologies.
  • Further research is needed to understand mechanisms and optimize delivery for various cell types and tissues.