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Related Experiment Videos

Piao Wei1, Yun Lv1, Qiao Guang1

  • 1Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, Guizhou Province, China.

Plant Signaling & Behavior
|June 8, 2023
PubMed
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Chicken interferon alpha (ChIFNα) promotes adventitious root development in Lotus japonicus by enhancing auxin signaling pathways. This study explores using animal genes for plant growth regulation in forage crops.

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Biotechnology

Background:

  • Adventitious roots (ARs) are crucial for plant development and propagation.
  • Understanding the molecular mechanisms regulating AR formation is vital for agricultural applications.
  • The role of animal-derived genes, like chicken interferon alpha (ChIFNα), in plant growth is largely unexplored.

Purpose of the Study:

  • To investigate the molecular mechanism by which ChIFNα influences AR differentiation in *Lotus japonicus*.
  • To determine if ChIFNα affects auxin pathways involved in root development.
  • To explore the potential of using ChIFNα for molecular breeding of forage plants.

Main Methods:

  • Generation and identification of ChIFNα transgenic *Lotus japonicus* plants (TP) using GUS staining, PCR, and RT-PCR.
Keywords:
Chicken interferon alpha geneLotus japonicus L.adventitious rootsauxin regulationforage plants

Related Experiment Videos

  • Quantification of recombinant ChIFNα (rChIFNα) using ELISA.
  • Analysis of root length, auxin (IAA) content, and enzyme activities (POD, PPO).
  • Transcriptome analysis to identify differentially expressed genes (DEGs) related to auxin pathways.
  • Validation of gene expression using RT-qPCR.

    • Main Results:

    • ChIFNα expression significantly promoted AR development and increased root length in transgenic plants.
    • Auxin (IAA) content and related enzyme activities (POD, PPO) were elevated in ChIFNα-expressing plants.
    • Transcriptome analysis identified 48 auxin-related DEGs, with GO enrichment highlighting the auxin pathway.
    • ChIFNα up-regulated genes involved in auxin synthesis and signaling, such as ALDH and GH3.
    • The effect of ChIFNα on AR development was further enhanced by auxin precursor (IBA) treatment.

    Conclusions:

    • ChIFNα promotes plant AR development by modulating auxin synthesis and signaling pathways.
    • This study demonstrates the efficacy of an animal-derived cytokine in regulating plant growth.
    • Findings provide insights into novel gene sources for molecular breeding and improving growth regulation in forage plants.