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Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Complete Genome Sequence of

Gyungcheon Kim¹, Jin Young Park², Jin Nam Kim³

¹Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, South Korea.

Microbiology Resource Announcements

|June 8, 2023

Summary

We sequenced the complete genome of Phenylobacterium sp. strain NIBR 498073, isolated from South Korean tidal flat sediment. This analysis provides foundational genomic data for this bacterial species.

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Last Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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Published on: March 15, 2019

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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Published on: August 20, 2021

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

Microbiology
Genomics

Background:

Phenylobacterium is a genus of bacteria found in various environments.
Genomic data is crucial for understanding bacterial diversity and function.

Purpose of the Study:

To report the complete genome sequence of Phenylobacterium sp. strain NIBR 498073.
To provide a foundational genomic resource for this strain.

Main Methods:

Whole genome sequencing of Phenylobacterium sp. strain NIBR 498073.
Genome annotation using the Prokaryotic Genome Annotation Pipeline (PGAP).

Main Results:

The complete genome sequence of Phenylobacterium sp. strain NIBR 498073 was determined.
The genome comprises a single circular chromosome of 4,289,989 base pairs.
Annotation predicted 4,160 protein-coding genes, 47 tRNAs, 6 rRNAs, and 3 noncoding RNAs.

Conclusions:

The genome sequence provides a comprehensive genetic blueprint for Phenylobacterium sp. strain NIBR 498073.
This data will aid future research into the biology and ecological role of this bacterium.