Highly Active CoRh Graphitic Nanozyme for Colorimetric Sensing in Real Samples
Phouphien Keoingthong1,2, Yiting Xu1,3, Shengkai Li1
1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, People's Republic of China.
The Journal of Physical Chemistry. B
|June 8, 2023
Summary
This study introduces a novel magnetic Rh nanozyme with a graphene shell, offering enhanced stability and superior peroxidase-like activity for sensitive colorimetric detection of dopamine and ascorbic acid.
Area of Science:
- Materials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Rh-based nanozymes offer high catalytic efficiency and stability.
- Magnetic nanozymes enable sensitive detection via magnetic separation.
- Stable magnetic Rh nanozymes have remained largely unreported.
Purpose of the Study:
- To develop a stable magnetic Rh nanozyme for sensitive colorimetric sensing.
- To investigate the catalytic activity and stability of the novel nanozyme.
- To demonstrate the application of the nanozyme in detecting dopamine and ascorbic acid.
Main Methods:
- Chemical vapor deposition (CVD) was used to synthesize CoRh graphitic nanozymes (CoRh@G).
- The nanozyme features a CoRh nanoalloy core encapsulated within a few layers of graphene.
- Peroxidase-like activity was evaluated using TMB oxidation, and detection of dopamine and ascorbic acid was performed colorimetrically.
Main Results:
- The CoRh@G nanozyme exhibited superior peroxidase-like activity and higher affinity for TMB oxidation than horseradish peroxidase (HRP).
- The graphitic shell provided excellent durability and recyclability to the nanozyme.
- High sensitivity and selectivity were achieved for the colorimetric detection of dopamine and ascorbic acid.
- The nanozyme demonstrated satisfactory performance in detecting ascorbic acid in commercial beverages.
Conclusions:
- The CoRh@G nanozyme is a stable, magnetic nanomaterial with excellent peroxidase-like activity.
- It offers a promising platform for sensitive and selective colorimetric detection of dopamine and ascorbic acid.
- The developed sensing platform holds potential for point-of-care (POC) visual monitoring applications.
