A Simple and Flexible Infusion Platform for Automated Native Mass Spectrometry Analysis

Area of Science:

• Biochemistry and Biophysics
• Analytical Chemistry
• Mass Spectrometry

Background:

• High-throughput native mass spectrometry (MS) of proteins and protein complexes relies on advanced infusion and liquid chromatography (LC) systems.
• Existing LC systems often possess underutilized gradient flow capabilities, presenting an opportunity for cost-effective adaptation.

Purpose of the Study:

• To demonstrate a lower-cost infusion cart for native mass spectrometry applications.
• To provide a versatile platform for both infusion and online buffer exchange experiments.
• To offer an affordable alternative for laboratories with limited budgets or for student training.

Main Methods:

• Development of an infusion cart utilizing a single isocratic solvent pump.
• The pump operates in both nano- and high-flow configurations, covering a range of 0.05-150 μL/min.
• The platform is controlled using open-source software, allowing for potential expansion and customization.

Main Results:

• Successful demonstration of a functional and cost-effective infusion cart for native MS.
• The system effectively supports both direct infusion and online buffer exchange experiments.
• The platform's flexibility in flow rates (0.05-150 μL/min) accommodates diverse experimental needs.

Conclusions:

• The developed infusion cart offers a significantly lower-cost alternative for native mass spectrometry.
• The platform's adaptability and open-source control enhance its utility for various laboratory settings.
• This innovation makes advanced native MS techniques more accessible to budget-conscious research groups and educational institutions.