Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is an established treatment suggestion in advanced-stage endometrial cancer fitting to all?

Taiwanese journal of obstetrics & gynecology·2026
Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026
Same author

Synthesis of an All-sp<sup>2</sup>-Hybridized Spheriphane C<sub>48</sub>H<sub>30</sub>.

Organic letters·2026
Same author

Workshop on Noninvasive Glucose Monitoring 2025.

Journal of diabetes science and technology·2026
Same author

Clinical Validation on Healthy Humans of a Portable Non-invasive Continuous Glucose Monitor Based on Transdermal Band-Pass Raman Spectroscopy.

Journal of diabetes science and technology·2026
Same author

Recurrent endometrial cancer: Suggesting treatment flowchart.

Taiwanese journal of obstetrics & gynecology·2026

Related Experiment Video

Updated: Jul 27, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.2K

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics.

Baoliang Ge1,2, Qing Zhang1, Rui Zhang3

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

ACS Photonics
|June 9, 2023
PubMed
Summary
This summary is machine-generated.

Polarized shearing interference microscopy (PSIM) enables quantitative measurement of molecular orientation dynamics in birefringent materials. This new method achieves high-speed imaging, overcoming previous limitations in dynamic material analysis.

Keywords:
birefringent structurepolarization microscopysingle-shot imaging

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.8K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.4K

Related Experiment Videos

Last Updated: Jul 27, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.2K
Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.8K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.4K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Biophysics

Background:

  • Polarization microscopy is crucial for analyzing molecular order in birefringent materials.
  • Existing techniques struggle with quantitative, millisecond-scale measurements of molecular orientation dynamics.
  • There is a need for advanced imaging methods to study dynamic changes in anisotropic materials.

Purpose of the Study:

  • Introduce polarized shearing interference microscopy (PSIM) as a novel single-shot quantitative polarization imaging technique.
  • Develop a method for precise measurement of retardance and orientation in complex birefringent structures.
  • Enable high-speed, quantitative analysis of molecular orientation dynamics.

Main Methods:

  • PSIM utilizes a single-shot quantitative polarization imaging approach.
  • The method extracts retardance and orientation angle of light transmitted through anisotropic specimens.
  • PSIM's performance was validated using a birefringent resolution target and bovine tendon.

Main Results:

  • PSIM accurately quantifies retardance and orientation in complex birefringent materials.
  • Demonstrated high-speed imaging of dynamic processes, achieving 506 frames per second.
  • Achieved a field-of-view up to 350 × 350 μm² with ~2 μm spatial resolution.

Conclusions:

  • PSIM overcomes limitations in measuring molecular orientation dynamics in birefringent materials.
  • The technique offers high-speed, quantitative imaging for complex structures.
  • PSIM is poised for broad applications in dynamic material characterization.