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Isolating Free Carbenes, their Mixed Dimers and Organic Radicals
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Berberine isolation from

R M Gamini Rajapakse1, Benjamin R Horrocks2, A U Malikaramage1

  • 1Department of Chemistry, Faculty of Science, University of Peradeniya Peradeniya 20400 Sri Lanka rmgr1521961@gmail.com.

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|June 9, 2023
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Summary
This summary is machine-generated.

Berberine from tree turmeric was studied using spectroscopy and quantum calculations. Its electronic properties are suitable for creating new donor-acceptor polymers.

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Area of Science:

  • Photochemistry
  • Computational Chemistry
  • Materials Science

Background:

  • Berberine, a natural alkaloid from Coscinium fenestratum (tree turmeric), possesses interesting photophysical properties.
  • Understanding berberine's electronic structure is crucial for its application in advanced materials.
  • Previous studies have explored berberine's biological activities, but its detailed electronic and electrochemical behavior requires further investigation.

Purpose of the Study:

  • To characterize the UV-Vis absorption and emission spectra of berberine in different media.
  • To computationally investigate the electronic transitions and charge transfer characteristics of berberine.
  • To determine the electrochemical gap and assess its potential for polymerization into donor-acceptor materials.

Main Methods:

  • Extraction and purification of berberine from Coscinium fenestratum using column chromatography.
  • UV-Vis absorption spectroscopy in acetonitrile and aqueous solutions.
  • Time-Dependent Density Functional Theory (TD-DFT) calculations with B3LYP functional.
  • Microelectrode voltammetry for electrochemical gap determination.
  • Quantum chemical calculations using cc-pVTZ basis set with B3LYP, CAM-B3LYP, and wB97XD functionals.

Main Results:

  • TD-DFT calculations accurately reproduced the main features of berberine's absorption and emission spectra.
  • Electronic transitions involve electron density transfer from the methylenedioxy phenyl ring to the isoquinolium moiety.
  • Electrochemical gap estimated at 2.64 V from voltammetry, showing good agreement with quantum chemical calculations.
  • Calculations indicate delocalized spin density in the radical dication of berberine.

Conclusions:

  • Berberine exhibits electronic properties consistent with a donor-acceptor system.
  • The calculated electronic transitions and electrochemical gap provide valuable data for material design.
  • Berberine is a promising candidate for synthesizing novel donor-acceptor polymeric materials via oxidative or co-polymerization.