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Updated: Jul 27, 2025

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
Beyond Natural Immune Repertoires: Synthetic Antibodies
Gianluca Veggiani1,2, Sachdev S Sidhu3
1The Anvil Institute, Kitchener, Ontario N2G 1H6, Canada.
Abstract:
Synthetic antibody libraries, in which the antigen-binding sites are precisely designed, offer unparalleled precision in antibody engineering, exceeding the potential of natural immune repertoires and constituting a novel generation of research tools and therapeutics. Recent advances in artificial intelligence-driven technologies and their integration into synthetic antibody discovery campaigns hold the promise to further streamline and effectively develop antibodies. Here, we provide an overview of synthetic antibodies. Our associated protocol describes how to develop highly diverse and functional synthetic antibody phage display libraries.
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