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LinShu Liu1, Adrienne B Narrowe1, Jenni A Firrman1

  • 1Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USA.

Foods (Basel, Switzerland)
|June 10, 2023
PubMed
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Laticaseibacillus rhamnosus strain GG (LGG) probiotics altered the gut metabolome in a human intestinal simulator. LGG promoted indole propionic acid production, a compound linked to improved human health.

Area of Science:

  • Microbiology
  • Metabolomics
  • Gastroenterology

Background:

  • The gut microbiome plays a crucial role in human health.
  • Understanding microbial metabolic functions is essential for developing targeted interventions.
  • Probiotics, like Laticaseibacillus rhamnosus strain GG (LGG), are known to influence gut microbial communities.

Purpose of the Study:

  • To investigate the impact of Laticaseibacillus rhamnosus strain GG (LGG) on the gut metabolome.
  • To identify specific metabolic pathways affected by LGG administration in a controlled in vitro setting.
  • To explore the relationship between microbial composition and metabolic output.

Main Methods:

  • Utilized a human intestinal microbial ecosystem simulator.
  • Administered Laticaseibacillus rhamnosus strain GG (LGG) to established microbial communities.
Keywords:
LGGLaticaseibacillus rhamnosus strain GGSHIME®gut microbiomeindole propionic acidmetabolome

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  • Performed shotgun metagenomic sequencing and metabolome analysis.
  • Employed an in vitro method for spatially-resolved metabolic profiling.

    • Main Results:

    • Observed correlations between microbial community shifts and altered metabolic output.
    • Identified sequential amino acid metabolic pathways along the colonic tract, with tryptophan and tyrosine derivatives produced distally.
    • Demonstrated that LGG administration promoted the production of indole propionic acid.
    • Indicated a potentially broader microbial community involved in indole propionic acid synthesis than previously recognized.

    Conclusions:

    • The study provides insights into the metabolic impact of Laticaseibacillus rhamnosus strain GG (LGG) in a simulated human colon.
    • Spatially-resolved metabolomics revealed distinct metabolic activities in different colonic regions.
    • The findings suggest LGG's potential to enhance the production of health-associated metabolites like indole propionic acid.