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Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection.

Elif Seymour1,2, Fulya Ekiz Kanik3, Sinem Diken Gür4

  • 1Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON M4P 1R2, Canada.

Sensors (Basel, Switzerland)
|June 10, 2023
PubMed
Summary
This summary is machine-generated.

Rapid, sensitive biosensors offer a faster alternative to Polymerase Chain Reaction (PCR) for diagnosing viral infections. Optical sensing techniques, like the single-particle interferometric reflectance imaging sensor (SP-IRIS), show promise for quick and accurate virus detection.

Keywords:
fluorescence-based sensorsinterferometric biosensorsoptical resonatorssingle-virus detectionsolid-phase optical biosensorssurface plasmon resonancevirus diagnostics

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Infectious Disease Diagnostics

Background:

  • Viral infections pose significant public health and economic threats, necessitating rapid and accurate diagnostic methods.
  • Current Polymerase Chain Reaction (PCR)-based virus detection methods are time-consuming and require specialized laboratory equipment, highlighting the need for advanced alternatives.
  • Biosensors offer potential for rapid, sensitive, and high-throughput viral diagnostics, crucial for disease control and mitigating societal impacts.

Purpose of the Study:

  • To review solid-phase optical sensing techniques for virus detection.
  • To highlight the advantages of optical biosensors for rapid and sensitive viral diagnostics.
  • To present the single-particle interferometric reflectance imaging sensor (SP-IRIS) as a novel digital virus detection platform.

Main Methods:

  • Review of various solid-phase optical sensing techniques including fluorescence, Surface Plasmon Resonance (SPR), Surface-Enhanced Raman Scattering (SERS), optical resonators, and interferometry.
  • Focus on interferometry-based biosensors, specifically the single-particle interferometric reflectance imaging sensor (SP-IRIS).
  • Demonstration of SP-IRIS for digital virus detection by visualizing single nanoparticles.

Main Results:

  • Optical sensing techniques offer high sensitivity and direct readout for virus detection.
  • The SP-IRIS sensor enables visualization of single nanoparticles, facilitating digital detection.
  • Interferometry-based platforms show significant potential for advanced viral diagnostics.

Conclusions:

  • Solid-phase optical biosensors represent a promising advancement in viral diagnostics, overcoming limitations of traditional methods.
  • The SP-IRIS platform offers a novel approach for sensitive and rapid digital virus detection.
  • Further development of these optical biosensing technologies is essential for effective public health surveillance and pandemic response.