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Spectroscopic Microtomography in the Short-Wave Infrared Wavelength Range.

Cory Juntunen1, Andrew R Abramczyk1, Peter Shea1

  • 1College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3200 N Cramer St., Milwaukee, WI 53211, USA.

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This study introduces a new 4D imaging technique using short-wave infrared (SWIR) spectroscopic microtomography. The method captures both chemical and structural information, enabling detailed analysis of microscopic samples.

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Area of Science:

  • Optical Imaging
  • Spectroscopy
  • Microscopy

Background:

  • 4D imaging combines 3D structural data with 1D chemical information.
  • Microscopic specimens require advanced imaging techniques for detailed analysis.

Purpose of the Study:

  • To demonstrate spectroscopic microtomography in the short-wave infrared (SWIR) range.
  • To capture both absorption coefficient and refractive index of microscopic samples.

Main Methods:

  • Utilized digital holographic tomography for imaging.
  • Employed a broadband laser with a tunable optical filter for wavelength scanning (1100-1650 nm).
  • Developed a system for 4D imaging of thick microscopic specimens.

Main Results:

  • Achieved a resolution of 1.51 μm (transverse) and 1.57 μm (axial) using gold nanoparticles.
  • Successfully imaged human hair and sea urchin embryos.
  • Demonstrated the capability to capture absorption coefficient and refractive index.

Conclusions:

  • The developed SWIR spectroscopic microtomography enables accurate and efficient 4D imaging.
  • This technique is suitable for specimens with distinct SWIR absorption or refractive index contrast.
  • Advances the field of microscopic imaging and chemical analysis.