Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reconstructive three-dimensional electron microscopy. A routine biologic tool.

J K Stevens, J Trogadis

    Analytical and Quantitative Cytology and Histology
    |June 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Rod and cone degeneration in the rd mouse is p53 independent.

    Molecular vision·2001
    Same author

    Adhesion of transplanted chondrocytes onto cartilage in vitro and in vivo.

    The Journal of rheumatology·2000
    Same author

    Factors affecting the efficacy of bovine chondrocyte transplantation in vitro.

    Osteoarthritis and cartilage·1999
    Same author

    Resistance of the dopamine D2L receptor to desensitization accompanies the up-regulation of receptors on to the surface of Sf9 cells.

    Endocrinology·1997
    Same author

    High-speed DNA sequencing in ultrathin slab gels.

    Current opinion in biotechnology·1997
    Same author

    Agonist-induced desensitization of dopamine D1 receptor-stimulated adenylyl cyclase activity is temporally and biochemically separated from D1 receptor internalization.

    Proceedings of the National Academy of Sciences of the United States of America·1995
    Same journal

    Micronuclei and other nuclear anomalies in buccal mucosa following exposure to X-ray radiation.

    Analytical and quantitative cytology and histology·2012
    Same journal

    Color deconvolution. Optimizing handling of 3D unitary optical density vectors with polar coordinates.

    Analytical and quantitative cytology and histology·2012
    Same journal

    Nuclear morphometry in the differential diagnosis of eosinophilic renal epithelial tumors.

    Analytical and quantitative cytology and histology·2012
    Same journal

    Spectrum of prostatic nonepithelial tumors and tumorlike conditions with spindle cell features.

    Analytical and quantitative cytology and histology·2012
    Same journal

    A framework for quantitative assessment of Ki67 distribution in preneoplastic bronchial epithelial lesions.

    Analytical and quantitative cytology and histology·2012
    Same journal

    Classification and grading of noninvasive and invasive neoplasms of the urothelium.

    Analytical and quantitative cytology and histology·2012
    See all related articles

    Serial electron microscopy now rapidly reconstructs cell structures, revealing new insights. This advanced technique has uncovered novel cellular features, such as the "nuclear tube" in mammalian nuclei.

    Area of Science:

    • Cell Biology
    • Microscopy Techniques

    Background:

    • Traditional serial electron microscopy was time-intensive, requiring over a year for data collection and analysis.
    • Significant technical advancements have drastically reduced the time needed for cellular reconstructions.

    Purpose of the Study:

    • To highlight the efficiency of modern serial electron microscopy for cellular analysis.
    • To showcase novel findings in cellular architecture enabled by these improved techniques.

    Main Methods:

    • Utilizing serial electron microscopy on embedded material.
    • Employing computer-aided reconstruction of cellular organelles and structures.
    • Implementing simple technical improvements to accelerate the process.

    Main Results:

    Related Experiment Videos

    • Complete computer reconstructions of cells' organelles and microtubules can now be generated in under a week.
    • Further improvements could reduce reconstruction time to two or three days.
    • New anatomical features, such as the "nuclear tube" in mammalian nuclei, have been discovered.

    Conclusions:

    • Serial electron microscopy, enhanced by technical improvements, is a powerful and efficient tool for exploring cellular structure and function.
    • These advancements provide unprecedented insights into three-dimensional cytoarchitecture.
    • The discovery of the "nuclear tube" exemplifies the potential for uncovering novel cellular components.