The correlation of molecule weight of chitosan oligomers with the corresponding viscosity and antibacterial activity

In order to explore the correlation between the viscosity of chitosan oligomers-acetic solution and its viscosity average molecular weight (M_v), and determine the M_v range with a strong bactericidal effect. A series of chitosan oligomers were obtained by degraded chitosan (728.5 kDa) with dilute acid and chitosan oligomer (101.5 kDa) was characterized by FT-IR, XRD, H NMR and C NMR. The bactericidal effect of chitosan oligomers with different Mv on E. coli, S. aureus and C. albicans was measured by plate counting method. And the bactericidal rate was taken as the evaluation indicator, the optimum conditions were determined by single-factor experiments. The result showed that the molecular structure of chitosan oligomers and original chitosan (728.5 kDa) were similar. The viscosity of the chitosan oligomers in acetic acid solution was positively correlated with the M_v, and the chitosan oligomers with the M_v of 52.5-145.0 kDa had a strong bactericidal performance. In addition, the bactericidal rate of chitosan oligomers on experimental strains was more than 90% when the concentration of 0.5 g/L (bacteria) and 1.0 g/L (fungi), pH6.0, incubation time of 30 min. Thus, chitosan oligomers had a potential application value when the M_v was in the range of 52.5-145.0 kDa.