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Updated: Jul 26, 2025
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Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells
Published on: April 21, 2021
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Retinal organoid and gene editing for basic and translational research.
You-Min Cheng1, Chao Ma1, Kangxin Jin1
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730 China.
Vision Research
|June 12, 2023
Summary
Retinal organoids and gene editing technologies are revolutionizing mammalian retina research. Their combination offers new avenues for studying retinal development, diseases, and potential therapies.
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Genetics
Background:
- Mammalian retina research is rapidly advancing due to breakthroughs in organoid technology and gene editing.
- Retinal organoids (ROs) closely mimic in vivo retinal development, offering a powerful model system.
Approach:
- This review explores recent innovations in generating diverse retinal organoids (ROs).
- It also covers advancements in gene editing tools like CRISPR-Cas9, prime editing, and HITI.
- The synergistic potential of combining ROs with gene editing for retinal studies is highlighted.
Key Points:
- Retinal organoids (ROs) replicate key molecular and cellular features of the native retina.
- Gene editing technologies, including CRISPR-Cas9 and its derivatives, enable precise genetic modifications.
- The integration of ROs and gene editing significantly expands research capabilities.
Conclusions:
- The convergence of ROs and gene editing presents unprecedented opportunities for understanding retinal biology.
- This powerful combination is poised to accelerate the development of novel therapeutic strategies for retinal diseases.
