Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.9K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.9K
DNA-only Transposons02:57

DNA-only Transposons

14.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.6K
Cleavage and Blastulation01:33

Cleavage and Blastulation

45.4K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
45.4K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.6K
Transposons01:24

Transposons

66
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
66
LTR Retrotransposons03:08

LTR Retrotransposons

17.6K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosome-level genome of diamondback terrapin provides insight into the genetic basis of salinity adaptation.

Integrative zoology·2024
Same author

Comparative analysis of the liver transcriptome in the red-eared slider (Trachemys scripta elegans) post exposure to noise.

PloS one·2024
Same author

Sleep disturbance in rodent models and its sex-specific implications.

Neuroscience and biobehavioral reviews·2024
Same author

The characterization of the mitochondrial genome of <i>Graptemys ouachitensis</i>.

Mitochondrial DNA. Part B, Resources·2024
Same author

The Characterization of Mitochondrial Genome of Spotted Pond Turtle (Geoclemys hamiltonii).

Biochemical genetics·2023
Same author

Comparing the efficacy and safety of low, medium, and high dosages of selexipag for treating pulmonary hypertension: A systematic review and meta-analysis.

Animal models and experimental medicine·2023

Related Experiment Video

Updated: Jul 26, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.5K

The composition dynamics of transposable elements in human blastocysts.

Jian Li1, Ping Yuan2,3, Guangwei Ma4

  • 1Department of Clinical Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Journal of Human Genetics
|June 12, 2023
PubMed
Summary

Transposable elements (TEs) proportions in human blastocysts are significantly influenced by parental genetic background and embryo development stage. These mobile DNA sequences show dynamic changes during early development.

More Related Videos

Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

22.6K
Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
04:04

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Published on: January 20, 2023

2.4K

Related Experiment Videos

Last Updated: Jul 26, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.5K
Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

22.6K
Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
04:04

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Published on: January 20, 2023

2.4K

Area of Science:

  • Genetics
  • Developmental Biology
  • Genomics

Background:

  • Transposable elements (TEs) are mobile DNA sequences crucial for genome evolution and regulation.
  • TEs play roles in embryo development and chromosomal structure, but their variation in human blastocysts is not fully understood.

Purpose of the Study:

  • To investigate the variation of transposable elements (TEs) in human blastocysts based on parental genetic background and developmental stage.
  • To identify specific TE subfamilies influenced by parental karyotype, blastocyst stage, and ploidy status.

Main Methods:

  • Analysis of 1137 TE subfamilies across six classes at the DNA level in 196 blastocysts.
  • Utilized Bowtie2 and PopoolationTE2 for TE proportion analysis.
  • Correlated TE frequencies with parental karyotype, blastocyst development stage, and ploidy status.

Main Results:

  • Parental karyotype was the primary factor influencing TE frequencies, with 1116 subfamilies showing variations.
  • Blastocyst development stage was the second most crucial factor, affecting 614 subfamilies.
  • Specific TE families like Alu and LINE showed distinct proportions at different blastocyst stages (e.g., Stage 6 and Stage 3).
  • TE subfamily proportions also varied with blastocyst karyotype (balanced vs. unbalanced), inner cell mass, and outer trophectoderm status.

Conclusions:

  • TE subfamily composition is dynamically modulated during human embryo development.
  • Parental genetic background and developmental stage significantly shape the TE landscape in blastocysts.
  • TE variations may serve as indicators of embryonic health and developmental potential.