Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

19.5K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.5K
Transgenic Plants02:50

Transgenic Plants

7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K
Transformation01:26

Transformation

36
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
36
The Central Dogma01:20

The Central Dogma

23.0K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
23.0K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.9K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.9K
Transgenic Organisms00:53

Transgenic Organisms

31.3K
Overview
31.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered plants for the production of the antioxidants arbutin and gallate.

Metabolic engineering·2025
Same author

Transcription factor binding divergence drives transcriptional and phenotypic variation in maize.

Nature plants·2025
Same author

Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.

The Plant journal : for cell and molecular biology·2024
Same author

Transcription factor binding site divergence across maize inbred lines drives transcriptional and phenotypic variation.

bioRxiv : the preprint server for biology·2024
Same author

History and current status of embryogenic culture-based tissue culture, transformation and gene editing of maize (Zea mays L.).

The plant genome·2024
Same author

A key to totipotency: Wuschel-like homeobox 2a unlocks embryogenic culture response in maize (Zea mays L.).

Plant biotechnology journal·2023

Related Experiment Video

Updated: Jul 26, 2025

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

23.4K

Maize transformation: history, progress, and perspectives.

Albert P Kausch1, Kan Wang2, Heidi F Kaeppler3

  • 1Department of Cell and Molecular Biology, University of Rhode Island, South Kingstown, RI 02892 USA.

Molecular Breeding : New Strategies in Plant Improvement
|June 13, 2023
PubMed
Summary
This summary is machine-generated.

Maize transformation technology has advanced significantly, improving genetic research and crop improvement. Future developments aim for genotype-independent transformation to boost global food security.

Keywords:
Advanced breedingFunctional genomicsGenetic modificationMorphogenic regulatorsPlant transformationZea mays

More Related Videos

Scalable Transfection of Maize Mesophyll Protoplasts
08:38

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

2.9K
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

12.6K

Related Experiment Videos

Last Updated: Jul 26, 2025

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

23.4K
Scalable Transfection of Maize Mesophyll Protoplasts
08:38

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

2.9K
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

12.6K

Area of Science:

  • Agricultural Science
  • Plant Biotechnology
  • Genomics

Background:

  • Maize genetic improvement relies heavily on functional genomics and transformation systems.
  • Maize transformation is a complex technology refined over decades through multidisciplinary efforts.
  • Advances in microbiology, DNA delivery, and plant regeneration have been crucial.

Purpose of the Study:

  • To review the historical development of maize transformation technology.
  • To provide a current perspective on key components influencing transformation efficiency.
  • To highlight future directions for maize transformation, particularly for genome editing applications.

Main Methods:

  • Historical review of maize transformation milestones and challenges.
  • Analysis of tissue culture, DNA delivery methods, and marker systems.
  • Discussion of growth-stimulating genes and genotype-specific improvements.

Main Results:

  • Significant progress has been made in optimizing maize transformation protocols.
  • CRISPR/Cas9 genome editing necessitates higher transformation efficiencies in key maize inbreds.
  • Current methods still face challenges in genotype independence and accessibility.

Conclusions:

  • Continued research in interdependent components is vital for maize transformation advancement.
  • Future maize transformation systems should be genotype-independent and widely accessible.
  • Enhanced transformation will accelerate genomics, genome editing, and breeding for global food security.